Food Funct. 2019;9(3-S):9726. Carbohydrate Polymers 72, 356-360. Intrinsic viscosity molecular weight relationship. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. J. Phys. Kumar SV, Tarun P, Kumar TA. Molecular weight determination of polysaccharides. Huggins, Maurice L. (1942). Sci. All authors read and approved the final manuscript. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. AATEX. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Stokes radius Microneedles and their applications. Physicochemical studies of oligodextran. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. European Polymer Journal, 37, 619-622. Regulating dynamic signaling between hematopoietic stem cells (2008). Determination of intrinsic viscosities of macromolecules and nanoparticles. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. Carbohydrate Polymers 88 373-381. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. ; Grishchenko, A.E. ; Dutt, N.V.K. Indo AM J Pharm. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Pharmacol Pharm. An undergraduate physical chemistry experiment. Molecular dynamics simulations on aqueous 1984;17(6):11703. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Dynamics of a Polymer Solution in a Rigid Matriz. Walkenhorst, S.; Olivier, R. (2003). The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. ; Hogan, M.P. Chem. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th A few percent of R (13) glucosidic linkages provides side chains which appear to be short. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Food Sci Biotechnol. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. (2013). Drug Deliv. Keywords: FITC dextran; intestinal permeability; transit time. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Ind. 2003;89(2):31120. (2000). After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Polymolecularity and polydispersity in Molecular weight determinations. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. This study was supported by the LG Electronics. CAS Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. Int J Pharm Investig. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. 2006;3(3):37385. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Google Scholar. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Correspondence to Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Optical properties of dextran in solution and in films. The genus Weissella: taxonomy, ecology and biotechnological potential. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Privacy 4h after microporation, the sizes of the micropore decreased slightly. Cookies policy. 2011;5(2):95132. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Small Intestinal Permeability and Gut-Transit Time Determined The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Herwadkar A, Banga AK. Polymer molecular weight distribution. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Learn More. 1905/05) for their financial support. MeSH terms Alternatives to animal testing: a review. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta De Belder, A.N. Dependence on Concentration. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. All authors have consented to the submission of this manuscript for publication. Degrees of long-chain branching in dextrans. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Thus, it is a common practice to express the frictional coefficient of rigid structures as. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). hydrodynamic pp13-21. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. Dextrans in Aqueous Solution. Experimental Review on Intrinsic If there are particles in the sample, the incident laser light gets scattered in all directions. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Granath, K. A. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. FITC-dextran solution was prepared at 1mM in PBS. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. 2008;26(11):12618. The Viscosity of Dilute Solutions of Long-Chain Molecules. Dilute solution properties and degree of chain branching for dextran. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. Springer Nature. II. dextran hydrodynamic radius Biophysical Journal, 87, 4259-4270. 2012;29(7):201729. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). ; De Jong, S.J. Search Biomaterials, 22, 1891-1898. Table 2 compares the properties of nanoparticles coated with one or other polymer. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. Drug Discov Today Technol. dextran The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Soc., 21, 1, 78-86. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. For data requests, please contact the authors. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. ; Ghosh, T.K. 2013;43(4):287303. Google Scholar. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Microporation was performed 2 times on each sample. After 24h, the cumulative permeation of FITC-dextran M.W. Journal of Physical Chemistry. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Ubbelohde, Leo. ; Garca de , J. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). dextran hydrodynamic Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data.
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dextran hydrodynamic radius